| 质粒名称: | M51 Super 8x FOPFlash (TOPFlash mutant) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 12457 |
| 存储实验室: | Randall Moon |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3 |
| 载体出品公司: | Promega |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 4839 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mutant TCF/LEF binding sites |
| 插入大小: | 134 bp |
| 基因突变: | mutant TCF/LEF binding sites |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Asp718(未破坏) |
| 3' 克隆位点: | XmaI (未破坏) |
| 5' 测序引物 | RVPrimer3 |
| 3' 测序引物 | LucNRev |
|
文献引用方法 |
|
| 材料和方法部分: | M51 Super 8x FOPFlash (TOPFlash mutant) was a gift from Randall Moon (Addgene plasmid # 12457) |
| 参考文献部分: | Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements. Veeman MT, Slusarski DC, Kaykas A, Louie SH, Moon RT. Curr Biol. 2003 Apr 15. 13(8):680-5. 10.1016/S0960-9822(03)00240-9 PubMed 12699626 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS M51 Super 8x FOPFlash (TOPFlash mutant) 4973 bp DNA SYN
DEFINITION M51 Super 8x FOPFlash (TOPFlash mutant)
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4973
/organism="M51 Super 8x FOPFlash (TOPFlash mutant)"
/mol_type="other DNA"
gene 243..1895
/label="firefly_Luciferase"
/gene="firefly_Luciferase"
CDS 243..1895
/label="ORF frame 3"
misc_feature complement(309..327)
/label="LucNrev_primer"
misc_feature complement(1947..1966)
/label="EBV_rev_primer"
gene complement(3235..4095)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(3235..4095)
/label="ORF frame 3"
promoter complement(4137..4165)
/label="AmpR_promoter"
rep_origin 4244..4550
/label="f1_origin"
CDS complement(4760..380)
/label="ORF frame 1"
misc_feature 4813..4966
/label="TB"
terminator 4813..4861
/label="synth_PA_terminator"
terminator 4813..4966
/label="up_PA_terminator"
misc_feature 4915..4934
/label="RVprimer3_primer"
ORIGIN
1 ggtaccttac gcgaggccaa agggggtaag gccaaagggg gtaaggccaa agggggtaag
61 gccaaaggcg cgaggccaaa gggggtaagg ccaaaggggg taaggccaaa gggggtaagg
121 ccaaaggccc gggctcgaga tctagactct agagggtata taatggaagc tcgaattcca
181 gcttggcatt ccggtactgt tggtaaaaag cttggcattc cggtactgtt ggtaaagcca
241 ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg
301 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa
361 ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt
421 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
481 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
541 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca
601 tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc
661 aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg
721 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg
781 attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg
841 gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct
901 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg
961 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat
1021 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt
1081 acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc
1141 tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct
1201 ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat
1261 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg
1321 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga
1381 aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg
1441 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt
1501 ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt
1561 ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc
1621 aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac
1681 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg
1741 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg
1801 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca
1861 taaaggccaa gaagggcgga aagatcgccg tgtaattcta gagtcggggc ggccggccgc
1921 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
1981 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
2041 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
2101 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg
2161 atccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
2221 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
2281 gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
2341 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
2401 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
2461 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
2521 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
2581 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
2641 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
2701 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
2761 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
2821 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
2881 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
2941 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
3001 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
3061 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
3121 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
3181 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
3241 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
3301 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
3361 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
3421 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
3481 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
3541 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
3601 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
3661 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
3721 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
3781 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
3841 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
3901 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
3961 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
4021 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
4081 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
4141 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
4201 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
4261 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
4321 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
4381 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
4441 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
4501 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
4561 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
4621 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
4681 ttcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
4741 gctattacgc cagcccaagc taccatgata agtaagtaat attaaggtac gggaggtact
4801 tggagcggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt
4861 gaatcgatag tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca
4921 aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctctatcg ata
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