M51 Super 8x FOPFlash (TOPFlash mutant)

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: M51 Super 8x FOPFlash (TOPFlash mutant) 
出品公司: 爱迪基因
目录编号: 12457
存储实验室: Randall Moon

载体骨架基本信息
载体名称: pGL3
载体出品公司: Promega
载体抗性: 氨苄霉素
空载体大小: 4839 bp
载体类型: 哺乳细胞表达
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: mutant TCF/LEF binding sites
插入大小: 134 bp
基因突变: mutant TCF/LEF binding sites
克隆方法: 限制性内切酶
5' 克隆位点: Asp718(未破坏)
3' 克隆位点: XmaI (未破坏)
5' 测序引物 RVPrimer3
3' 测序引物 LucNRev

文献引用方法
材料和方法部分: M51 Super 8x FOPFlash (TOPFlash mutant) was a gift from Randall Moon (Addgene plasmid # 12457)
参考文献部分: Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements. Veeman MT, Slusarski DC, Kaykas A, Louie SH, Moon RT. Curr Biol. 2003 Apr 15. 13(8):680-5. 10.1016/S0960-9822(03)00240-9 PubMed 12699626

载体图谱质粒图谱和多克隆位点信息

M51 Super 8x FOPFlash (TOPFlash mutant)载体图谱

载体简介

载体序列

LOCUS       M51 Super 8x FOPFlash (TOPFlash mutant)	4973 bp 	DNA	SYN
DEFINITION  M51 Super 8x FOPFlash (TOPFlash mutant)
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="M51 Super 8x FOPFlash (TOPFlash mutant)"
                     /mol_type="other DNA"
     gene            243..1895
                     /label="firefly_Luciferase"
                     /gene="firefly_Luciferase"
     CDS             243..1895
                     /label="ORF frame 3"
     misc_feature    complement(309..327)
                     /label="LucNrev_primer"
     misc_feature    complement(1947..1966)
                     /label="EBV_rev_primer"
     gene            complement(3235..4095)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(3235..4095)
                     /label="ORF frame 3"
     promoter        complement(4137..4165)
                     /label="AmpR_promoter"
     rep_origin      4244..4550
                     /label="f1_origin"
     CDS             complement(4760..380)
                     /label="ORF frame 1"
     misc_feature    4813..4966
                     /label="TB"
     terminator      4813..4861
                     /label="synth_PA_terminator"
     terminator      4813..4966
                     /label="up_PA_terminator"
     misc_feature    4915..4934
                     /label="RVprimer3_primer"
ORIGIN
    1 ggtaccttac gcgaggccaa agggggtaag gccaaagggg gtaaggccaa agggggtaag
   61 gccaaaggcg cgaggccaaa gggggtaagg ccaaaggggg taaggccaaa gggggtaagg
  121 ccaaaggccc gggctcgaga tctagactct agagggtata taatggaagc tcgaattcca
  181 gcttggcatt ccggtactgt tggtaaaaag cttggcattc cggtactgtt ggtaaagcca
  241 ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg
  301 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa
  361 ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt
  421 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
  481 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
  541 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca
  601 tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc
  661 aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg
  721 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg
  781 attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg
  841 gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct
  901 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg
  961 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat
 1021 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt
 1081 acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc
 1141 tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct
 1201 ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat
 1261 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg
 1321 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga
 1381 aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg
 1441 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt
 1501 ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt
 1561 ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc
 1621 aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac
 1681 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg
 1741 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg
 1801 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca
 1861 taaaggccaa gaagggcgga aagatcgccg tgtaattcta gagtcggggc ggccggccgc
 1921 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
 1981 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
 2041 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
 2101 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg
 2161 atccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
 2221 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
 2281 gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
 2341 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
 2401 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
 2461 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
 2521 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
 2581 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
 2641 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
 2701 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
 2761 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
 2821 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
 2881 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
 2941 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
 3001 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
 3061 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
 3121 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
 3181 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
 3241 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
 3301 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
 3361 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
 3421 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
 3481 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
 3541 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
 3601 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
 3661 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
 3721 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
 3781 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
 3841 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
 3901 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
 3961 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
 4021 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
 4081 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
 4141 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
 4201 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
 4261 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
 4321 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
 4381 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
 4441 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
 4501 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
 4561 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
 4621 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
 4681 ttcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
 4741 gctattacgc cagcccaagc taccatgata agtaagtaat attaaggtac gggaggtact
 4801 tggagcggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt
 4861 gaatcgatag tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca
 4921 aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctctatcg ata

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